Open interdental spaces caused by papillary gingival recession are one of the most common problems faced in dentistry. Surgical and nonsurgical periodontal treatments for regeneration of lost papillary height have been reported with limited success. The present study reports effectiveness of autologous cultured fibroblast injections, a tissue engineering technique for papillary regeneration. A black triangle caused by Tarnow's and Nordland's class I papillary gingival loss was reported in maxillary anterior region of a young male patient. An autologous gingival biopsy was cultured in a biotechnology lab for the growth and expansion of fibroblasts. Cultured fibroblast suspension was injected into the receded papilla twice at an interval of 5 days. Follow-ups were recorded on the 6th day, 15 ^th day, at 1 month and at 2 months. Complete fill of black triangle was noted at the end of 2 months. No inflammatory or immune reactions were noted at the site of injection. Autologous cultured fibroblast injections are safe, efficacious, and an acceptable treatment option for the regeneration of lost papillary height.

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Aims and Objectives: The aim of this work was to compare the fracture resistance values of "Mesio-occlusal" preparation, "slot" preparation with "conventional grooves," "box-only," and "MOD" preparation. Materials and Methods: A total of 60, noncarious extracted maxillary premolars were selected and divided into four groups, consisting of 15 teeth each -- group A: "Mesio-occlusal" preparation, group B: "slot" preparation (conventional grooves), group C: "box-only" preparation (long grooves), group D: "MOD" cavity preparation. All the specimens were subjected to compressive axial load in triaxial testing machine at a cross head speed of 1.2 mm/min at an inclination of 13.5°. Results: Group A ("Mesio-occlusal") preparation had the least fracture resistance value and group D ("MOD") cavity preparation had the highest fracture resistance. There was a significant difference in fracture load found between groups B and C. Conclusion: Amalgam restorations with "Mesio-occlusal" preparations had the least fracture resistance. Amalgam restorations with "slot" preparations had better fracture resistance than "box-only" preparation. Amalgam restorations with "MOD" preparations had the highest fracture resistance.

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Pleomorphic adenoma is the most common benign salivary gland tumor, which has elements of both epithelial and mesenchymal tissues. The tumor most commonly arises in the parotid or submandibular glands. Infrequently, it may arise from the minor salivary glands. We report a case of pleomorphic adenoma of minor salivary gland of palate in an 18-year-old young female patient for which the surgical treatment was rendered and with no evidence of recurrence.

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Aim: The purpose of this review was to evaluate the effectiveness of photodynamic therapy (PDT) for periodontitis. This review also elucidates application of photodynamic therapy for noninvasive management of periodontitis without leading to bacterial resistance. Background: Periodontal diseases are one of the major causes of tooth loss in adults and are considered primarily an anaerobic bacterial infections caused by the so-called red complex species. Bacteria present in a biofilm community, enzymes, endotoxins, and other cytotoxic factors lead to tissue destruction and initiate chronic inflammation. Since many years pioneers have been working to provide logical and cost-effective therapy for management of periodontitis. Periodontal researchers have found that PDT is advantageous to suppress anaerobic bacteria. Clinical Significance: Applications of PDT in dentistry are growing rapidly. PDT application has an adjunctive benefit besides mechanical treatment at sites with difficult access. Necessity for flap surgery may be reduced, patient comfort may increase, and treatment time may decrease. The application of photosensitizing dyes and their excitation by visible light enables effective killing of periodonto-pathogens. The introduction of laser along with photosensitizers has brought a revolutionary change. Conclusion: The application of photodynamic therapy in management of periodontal diseases is very valuable. The therapy should be combined with nonsurgical periodontal therapy. Proper clinical application of photodynamic therapy can and will help patients who are systemically compromised and cannot undergo surgical therapy.

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Hard and soft tissue alveolar ridge deformities occur following the extraction of teeth, creating functional and esthetic tooth replacement challenges, especially in the esthetic zone. A surgical technique using a roll flap technique for ridge augmentation is discussed which when combined with conventional fixed partial dentures for tooth replacement creates a template of the labial and interproximal sulcus at the edentulous site, allowing for the molding of an ovate pontic resulting in greatly improved esthetics in an esthetically compromised case. This results in vastly improved restorative emergence profiles with minimal or no change in the height of the labial gingival margin and the form and height of the interproximal papillae with adjacent teeth. This technique enhances soft tissue contours adjacent to the definitive restoration that vary little from those of the original natural tooth.

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Aim: This study was undertaken to compare the sealing ability of bone cement as furcation perforation repair material when compared with mineral trioxide aggregate and calcium phosphate cement. Materials and Methods: A total of 70 sound mandibular molars were selected for this study. The sample teeth were randomly divided into five groups: group I - n=20, perforation repair material used, mineral trioxide aggregate; group II - n=20, perforation repair material used, calcium phosphate cement; group III - n=20, perforation repair material used, bone cement; group IV - positive control, n=5, the furcation were not repaired with any material; group V - negative control, n=5, furcation area intact, no perforation done. The teeth were immersed in silver nitrate solution for 2 hours and then rinsed with photographic developer solution for 6 hours. They were then sectioned in a longitudinal direction and examined under a stereomicroscope. In each section the actual values of dye leakage were calculated from outer margins of perforation to the level of pulpal floor and were then subjected to statistical analysis. Results: An unpaired 't' test revealed that different groups exhibited significantly different dye penetrations (P<0.01). Conclusion: Furcation perforation repaired with MTA showed minimum microleakage (mean 54.5%), calcium phosphate cement showed maximum microleakage (100%), and bone cement showed moderate dye leakage (87.8%).

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Background: Actinobacillus actinomycetemcomitans is thought to be one of the etiological agents in aggressive periodontitis as well as indicated in various systemic diseases. Objective: To evaluate the prevalence of A. actinomycetemcomitans in the subgingival plaque of aggressive periodontitis patients. Study Design: Initially, under the selective growth conditions, the isolates were picked from the plaques and their identification was confirmed by polymerase chain reaction using primers specific for A. actinomycetemcomitans subgingival plaque of 15 patients diagnosed clinically and on radiographic criteria as aggressive periodontitis was inoculated on the Tryptic Soy agar with Bacitracin and Vancomycin culture media for 3-5 days under microaerophilic conditions. The positive colonies were selected based on biochemical tests for further analysis using reported primers for A. actinomycetemcomitans. Results: The results showed that 66.67% of aggressive periodontitis patients and 6.67% of control group of normal patients showed evidence of presence of A. actinomycetemcomitans in the subgingival microflora. Conclusion: This is the first study of its kind in an Indian population whereby almost all aggressive periodontitis patients showed evidence of A. actinomycetemcomitans.

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Odontogenic keratocyst is a unique cyst because of its locally aggressive behavior, high recurrence rate, and characteristic histological appearance. In this case report we present a 25-year-old male patient with a large odontogenic keratocyst and treatment with enucleation and chemical cauterization followed by dental implants with a 7-year follow-up.

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The peripheral ossifying fibroma (POF) is an exophytic gingival mass of fibrous connective tissue covered with a surface epithelium associated with the formation of randomly dispersed foci of a mineralized product consisting of bone, cementum-like tissue, or dystrophic calcifications having a recurrent rate of nearly 20%. It is one of the most common reactive gingival lesions, which have often been called by the generic term "epulis." This case report describes the clinical and histopathological findings of POF, its differential diagnosis, and treatment.

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