JICDRO is a UGC approved journal (Journal no. 63927)
Year : 2022  |  Volume : 14  |  Issue : 1  |  Page : 52-59

A novel method of decalcification of bone by heat-accelerated method with tissue floatation bath using nitric acid and formic Acid: A comparative study

1 Department of Oral Pathology and Microbiology, Rural Dental College, Pravara Institute of Medical Sciences, Loni, Ahmednagar, Maharashtra, India
2 Department of Oral Pathology and Microbiology, MGV's KBH Dental College, Nashik, Maharashtra, India

Correspondence Address:
Dr. Amruta Hiraman Galphade
PIMS, PMT Campus, Gate No. 1, Loni, Maharashtra
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jicdro.jicdro_80_21

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Context: Decalcification of bone is a challenging aspect since penetration of decalcifying agents through dense alveolar bone is a slow process. Striking the right balance between speed and quality of staining is essential for accurate and timely diagnosis of bone pathologies. Aims: This study aimed to compare the rate of decalcification of bone by employing conventional method and tissue floatation bath (TFB) using 10% nitric acid and 10% formic acid. It also compared the cellular and staining characteristics of decalcified specimens of bone by the abovementioned methods. Materials and Method: Forty bone slices of hemimandibulectomy specimens were decalcified by employing conventional method and TFB at elevated temperature using 10% nitric acid and 10% formic acid. Following evaluation by chemical endpoint test, they were then subjected to tissue processing and staining with hematoxylin and eosin stain and assessed for various parameters. Statistical Analysis Used: Statistical analysis was done using statistical software SPSS version 18.0. One-way ANOVA was applied to evaluate the significant differences among the mean values in different groups. Results: The heat-accelerated TFB method significantly reduced the time of decalcification of bone without compromising the cellular and nuclear details. The cellular and staining characteristics of TFB method were better than those decalcified by conventional method. Conclusions: TFB method using 10% formic acid at elevated temperature provided the best cellular and nuclear details with reduced decalcification time as compared to conventional methods.

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