JICDRO is a UGC approved journal (Journal no. 63927)
Year : 2022  |  Volume : 14  |  Issue : 1  |  Page : 37-43

The effect of different irrigants on sealer penetration into dentinal tubules with and without activation, using confocal scanning microscope

Department of Endodontics, Riyadh Elm University, Riyadh, Kingdom of Saudi Arabia

Correspondence Address:
Dr. Shibu Thomas Mathew
Department of Endodontics, Riyadh Elm University, Riyadh
Kingdom of Saudi Arabia
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jicdro.jicdro_20_21

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Purpose: To compare the effectiveness of three root canal irrigants with the influence of activation technique on the depth of sealer penetration into dentinal tubules using a confocal laser scanning microscope (CLSM). Materials and Methods: Sixty recently extracted human mandibular premolars with single straight canals (n = 60) were prepared with ProTaper Next NiTi rotary files up to X3. The samples were divided into three groups according to the final irrigation solution (n = 20), 5.25% sodium hypochlorite, 2% chlorhexidine digluconate, 17% ethylene-diamine-tetraacetic acid (EDTA). Each group was further subdivided into two groups according to the ultrasonic activation protocol and then obturated with a single gutta-percha size X3 and labeled sealer mixed with 0.01% fluorescent rhodamine B after the final irrigation protocol. Transverse sections were obtained at 2, 5, and 8 mm from the apex and observed under CLSM. It recorded the maximum penetration depth of both irrigant and sealer. Data were analyzed using a three-way analysis of variance and Tukey test (P = 0.05). Results: 17% EDTA exhibited significantly higher penetration depth with ultrasonic activation than the other groups and also determined (P < 0.011) statistically significant differences at root canal third (coronal, middle, apical; P < 0.011). Conclusions: The dentinal tubule penetration was significantly affected by selecting a root canal irrigant, an ultrasonic activation protocol, and root canal third. 17% EDTA with ultrasonic activation seemed beneficial in dentinal tubule penetration. Hence, the depth of sealer penetration might influence the outcome or success rate of endodontic therapy.

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