JICDRO is a UGC approved journal (Journal no. 63927)
Year : 2014  |  Volume : 6  |  Issue : 1  |  Page : 16-19

Recent advances in periodontal microbiology: An update on cultivation techniques

Department of Microbiology and Molecular Biology, Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre, Belgaum, Karnataka, India

Correspondence Address:
Kishore G Bhat
Department of Microbiology and Molecular Biology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belgaum - 590 010, Karnataka
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2231-0754.139087

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Microbial members of the subgingival plaque community play a major role in the initiation and progression of periodontal diseases. Majority of these bacteria are anaerobic in nature and several anaerobic systems have been used for their cultivation. Among them anaerobic jars are the most popular and are routinely used for the detection of periodontal pathogens from clinical samples. Despite best efforts, a significant portion of oral microbes have not yet been cultivated and several hypotheses have been put forth to explain this anomaly. This has led to renewed efforts to cultivate the oral bacteria so far identified only by their molecular signatures resulting in improvisation of existing culture techniques and devising novel methods of isolation. Several devices have been used on environmental samples successfully: One method called "minitrap" has been successfully adapted to oral cavity and has shown great promise in isolation of not yet cultivated oral bacterial species. These newer techniques are sure to shed more light on the role of microbes in the etiology of periodontal diseases.

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